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ScreenFect GmbH tcf firefly luciferase top-flash
Tcf Firefly Luciferase Top Flash, supplied by ScreenFect GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tcf+firefly+luciferase+top-flash/tcf+firefly+luciferase+top+flash/pmc12153800-63-26-44
Average 90 stars, based on 1 article reviews
tcf firefly luciferase top-flash - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Activity Assay:

Article Title: Quantification of Wnt3a, Wnt5a and Wnt16 Binding to Multiple Frizzleds Under Physiological Conditions Using NanoBit/BRET
Article Snippet: To test the biological activity of eGFP-Wnt-3a, eGFP-Wnt-5a and eGFP-Wnt-16, 5.5 × 10 4 HEK293T cells cultured in 96-well plates were transfected with 20 ng of TCF firefly luciferase (TOP-FLASH), 2 ng of CMV Renilla luciferase and 78 ng of pCS2+ empty plasmid using ScreenFect ® A according to the manufacturer’s 1-step protocol (ScreenFect GmbH, Eggenstein-Leopoldshafen, Germany).

Cell Culture:

Article Title: Quantification of Wnt3a, Wnt5a and Wnt16 Binding to Multiple Frizzleds Under Physiological Conditions Using NanoBit/BRET
Article Snippet: To test the biological activity of eGFP-Wnt-3a, eGFP-Wnt-5a and eGFP-Wnt-16, 5.5 × 10 4 HEK293T cells cultured in 96-well plates were transfected with 20 ng of TCF firefly luciferase (TOP-FLASH), 2 ng of CMV Renilla luciferase and 78 ng of pCS2+ empty plasmid using ScreenFect ® A according to the manufacturer’s 1-step protocol (ScreenFect GmbH, Eggenstein-Leopoldshafen, Germany).

Transfection:

Article Title: Quantification of Wnt3a, Wnt5a and Wnt16 Binding to Multiple Frizzleds Under Physiological Conditions Using NanoBit/BRET
Article Snippet: To test the biological activity of eGFP-Wnt-3a, eGFP-Wnt-5a and eGFP-Wnt-16, 5.5 × 10 4 HEK293T cells cultured in 96-well plates were transfected with 20 ng of TCF firefly luciferase (TOP-FLASH), 2 ng of CMV Renilla luciferase and 78 ng of pCS2+ empty plasmid using ScreenFect ® A according to the manufacturer’s 1-step protocol (ScreenFect GmbH, Eggenstein-Leopoldshafen, Germany).

Luciferase:

Article Title: Quantification of Wnt3a, Wnt5a and Wnt16 Binding to Multiple Frizzleds Under Physiological Conditions Using NanoBit/BRET
Article Snippet: To test the biological activity of eGFP-Wnt-3a, eGFP-Wnt-5a and eGFP-Wnt-16, 5.5 × 10 4 HEK293T cells cultured in 96-well plates were transfected with 20 ng of TCF firefly luciferase (TOP-FLASH), 2 ng of CMV Renilla luciferase and 78 ng of pCS2+ empty plasmid using ScreenFect ® A according to the manufacturer’s 1-step protocol (ScreenFect GmbH, Eggenstein-Leopoldshafen, Germany).

Plasmid Preparation:

Article Title: Quantification of Wnt3a, Wnt5a and Wnt16 Binding to Multiple Frizzleds Under Physiological Conditions Using NanoBit/BRET
Article Snippet: To test the biological activity of eGFP-Wnt-3a, eGFP-Wnt-5a and eGFP-Wnt-16, 5.5 × 10 4 HEK293T cells cultured in 96-well plates were transfected with 20 ng of TCF firefly luciferase (TOP-FLASH), 2 ng of CMV Renilla luciferase and 78 ng of pCS2+ empty plasmid using ScreenFect ® A according to the manufacturer’s 1-step protocol (ScreenFect GmbH, Eggenstein-Leopoldshafen, Germany).



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